A marvellous Luis Alberto volley cancelled out the Bryan Linssen opener, as Lazio drew 1-1 with Vitesse and fumbled their perfect record.
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The Aquile had already booked their spot in the Round of 32 as group winners, being the only side in the competition to have won the opening four games. They are joined by Nice, who beat Zulte Waregem 3-1 with a Mario Balotelli brace.
Simone Inzaghi rang the changes and wanted a reaction after Saturday’s Derby della Capitale defeat to Roma ended the run of nine consecutive victories in all competition. Vitesse, who lost 3-2 back in September, needed a victory and to hope Nice drew with Zulte Waregem to stay in the running.
Tim Matavz drilled inches wide of the near post within four minutes and Bryan Linssen broke the deadlock with a scorching touched-on free kick that bounced off the underside of the bar, leaving Ivan Vargic with little chance.
Vargic was ready for a ferocious long-range Mason Mount strike, palming it out from under the bar, and Navarone Foor blasted over from distance.
Lazio almost equalised with a spectacular team move, as Luis Alberto’s back-heel flick set up Simone Palombi and Remko Pasveer flew to beat away the curling finish.
Luis Alberto did get them on level terms with a wonderful low volley at the near bottom corner with the inside of his right boot on a floated Dusan Basta cross from the right.
There was bad news for the hosts straight after the restart, as Luis Nani suddenly pulled up clutching his right thigh and had to be substituted.
Pasveer smothered a Luca Crecco snapshot, Luc Castaignos nodded a corner over the bar and Senad Lulic saw his deflection from a set play palmed on to the side-netting.
There was time for Alessio Miceli to make his senior professional debut and he got back to intercept a very dangerous cross before it could reach Linssen.
Lazio 1-1 Vitesse
Linssen 13 (V), Luis Alberto 43 (L)
Lazio: Vargic; Patric, Luiz Felipe, Bastos; Basta (Miceli 85), Murgia, Luis Alberto (Marusic 68), Crecco, Lukaku; Nani (Lulic 54); Palombi
Vitesse: Pasveer; Lelieveld (Dabo 81), Kashia, Miazga, Faye; Serero, Ali, Mount, Foor; Rashica (Van Bergen 68), Matavz (Castaignos 61), Linssen
Ref: Ali Palabıyık (TUR)