Milan have named Alexandre Pato, Alessandro Nesta and Christian Abbiati in their squad to face Barcelona.
There were doubts over the fitness levels of these three players, but all are included along with Ignazio Abate and Kevin-Prince Boateng, who made their comebacks over the last week.
Pato has been out of action for many months, but received reassurance during a specialist consultation in Atlanta, Georgia.
The Rossoneri travel to the Camp Nou on Tuesday evening for the Champions League quarter-final, as the first leg ended 0-0.
It has also been confirmed that Dutch referee Bjorn Kuipers takes charge of the game.
Milan squad:Abbiati, Amelia, Piscitelli, Abate, Antonini, Bonera, De Sciglio, Mesbah, Mexes, Nesta, Zambrotta, Yepes, Ambrosini, Aquilani, Boateng, Emanuelson, Nocerino, Seedorf, El Shaarawy, Ibrahimovic, Maxi Lopez, Pato, Robinho