Paul Pogba stands in for Andrea Pirlo, while Miroslav Klose and Antonio Candreva are extra motivated as Lazio visit Juventus at 17.00 GMT.
The huge clash is between the Serie A leaders and the side currently in fifth place, who are fresh from winning the Derby della Capitale.
Juve have comfortably won both games since their 49-match unbeaten Serie A streak was ended here by Inter, but must do without star midfielder Pirlo.
The veteran is suspended, so makes way for Pogba after he was dropped for turning up late to training last week.
Mirko Vucinic is also out, as he left the camp this morning after coming down with flu symptoms, so Fabio Quagliarella partners Sebastian Giovinco.
Mauricio Isla is again picked to start in place of out-of-favour Stephan Lichtsteiner.
Lazio also have to deal with absentees, as Senad Lulic and Stefano Mauri both sit out bans with Modibo Diakite, Francelino Matuzalem and Giuseppe Sculli unavailable.
Klose is eager to score his first Serie A goal against Juventus, while Candreva is motivated by the fact he played for the Bianconeri and they did not take up their option to make the loan permanent.
Juve must also take into account Tuesday’s Champions League showdown with Chelsea.
Juventus: Buffon; Barzagli, Bonucci, Chiellini; Isla, Vidal, Pogba, Marchisio, Asamoah; Giovinco, Quagliarella
Juventus bench: Storari, Rubinho, Caceres, Lichtsteiner, Lucio, De Ceglie, Padoin, Marrone, Giaccherini, Pepe, Bendtner, Matri
Lazio: Marchetti; Konko, Biava, Dias, Radu; Candreva, Hernanes, Ledesma, Brocchi, Gonzalez; Klose
Lazio bench: Bizzarri, Carrizo, Cavanda, Stankevicius, Ciani, Onazi, Ederson, Cana, Kozak, Zarate, Floccari, Rocchi