Mario Gomez and Joaquin aim to send Fiorentina through to the Europa League against Grasshoppers.
The Viola already won the first leg 2-1 in Zurich, but the decider kicks off at the Stadio Artemio Franchi at 20.00 UK time (19.00 GMT).
Gomez missed a couple of sitters in his first two competitive matches for Fiorentina, so will be eager to break his duck.
Giuseppe Rossi already netted his first goal in two years during the 2-1 Serie A victory over Catania on Monday night, but is rested as he continues to recover from a devastating knee injury.
Instead, summer signing Joaquin is given an opportunity to shine alongside Gomez, while Borja Valero, Mati Fernandez and David Pizarro complete the midfield.
Grasshopper go for Ben Khalifa from the start partnering Ngakumol, while Salatic protects the back three in a mirror-image of Fiorentina’s tactics.
Fiorentina: Neto; Tomovic, Gonzalo Rodriguez, Savic; Pizarro; Cuadrado, Borja Valero, Fernandez, Pasqual; Joaquin, Gomez
Fiorentina bench: Munua, Alonso, Compper, Bakic, Aquilani, Ilicic, Rossi
Grasshopper: Burki: M Lang, Grichting, Vilotic; Salatic; Gashi, Abrashi, Toko, Pavlovic; Ben Khalifa, Ngamukol
Grasshopper bench: Taini, Bauer, Feltscher, I Hajrovic, S Lang, Caio, Vonlanthen