Lazio aim to put their Derby woes behind them against Catania, a team with just one point so far this season.
It kicks off at the Stadio Olimpico at 19.45 UK time (18.45 GMT), click here for a match preview.
Vladimir Petkovic insisted Lazio were “already over” their 2-0 defeat to arch rivals Roma, though it left plenty of problems in its wake.
Miroslav Klose, Stefan Radu, Giuseppe Biava, Abdoulay Konko, Lucas Biglia and Diego Novaretti are out injured with Andre Dias and Stefano Mauri suspended.
It forces big changes for the Biancocelesti, including the debut Serie A start for Bruno Pereirinha and Sergio Floccari as the lone striker, as Hernanes is rested.
Catania have only one point from four rounds, terminating their negative streak with a 0-0 home draw against Parma on Sunday.
Mariano Izco, Sebastian Leto and Lucas Castro are out of action.
They start with summer signing Fabian Monzon and Bruno Petkovic – no relation to the Lazio Coach! He just turned 19 last week and is making his Serie A debut.
Lazio: Marchetti; Cavanda, Ciani, Cana, Pereirinha; Candreva, Ledesma; Onazi, Ederson, Lulic; Floccari
Catania: Andujar; Alvarez, Bellusci, Spolli, Biraghi; Plasil, Almiron, Guarente, Monzon; Barrientos, Petkovic