Lazio and Fiorentina go into a battle for European qualification, as Giuseppe Rossi returns to face the Aquile.
It kicks off at the Stadio Olimpico at 19.45 UK time (18.45 GMT), click here for a match preview.
Both sides are tired from Thursday’s difficult Europa League away games, as Lazio drew 3-3 with Trabzonspor and the Viola beat Dnipro 2-1 under the snow.
They also have to deal with numerous injury problems, as Vladimir Petkovic missed Miroslav Klose, Abdoulay Konko, Giuseppe Biava, Stefan Radu, Bruno Pereirinha and the suspended Stefano Mauri.
Despite his brace in Turkey, Sergio Floccari is again benched in favour of 20-year-old South Americans Felipe Anderson and Brayan Perea.
Fiorentina are without Mario Gomez and Ante Rebic, but Rossi has shaken off his muscular injury to start.
Pepito limped off during Monday’s 2-2 draw with Parma, but is fit and ready to celebrate his recall to the Italy squad after two years.
Lazio: Marchetti; Cavanda, Ciani, Cana, Lulic; Biglia, Hernanes; Candreva, Felipe Anderson, Onazi; Perea
Lazio bench: Berisha, Strakosha, Dias, Campos, Keita, Gonzalez, Elez, Ledesma, De Freitas
Fiorentina: Neto; Tomovic, Gonzalo, Savic, Pasqual; Ambrosini, Pizarro, Aquilani; Borja Valero, Cuadrado; Rossi
Fiorentina bench: Munua, Alonso, Roncaglia, Compper, Bakic, Mati Fernandez, Joaquin, Vecino, Wolsky, Matos, Vargas, Iakovenko