Napoli have Gonzalo Higuain, Dries Mertens and Goran Pandev to take on Olympique Marseille.
The Partenopei earned a 2-1 victory in France last month and are joint top of Group F with Arsenal and Borussia Dortmund.
They host this tie at 19.45 GMT at the Stadio San Paolo against an OM side without a single point so far.
Rafa Benitez has to do without Juan Camilo Zuniga, Giandomenico Mesto and Miguel Angel Britos.
Higuain leads the attack with support from Jose Maria Callejon, Mertens and Pandev, while Lorenzo Insigne is ready to bring fresh legs in the second half.
Marek Hamsik is surprisingly dropped to the bench with Blerim Dzemaili preferred to Valon Behrami in midfield.
Once again Paolo Cannavaro is dropped from the defence in favour of Federico Fernandez, while Christian Maggio is fit again at right-back.
Marseille are in crisis, though a 1-1 draw at Stade Rennais at the weekend terminated a run of five consecutive defeats.
They are without defenders Lucas Mendes and Rod Fanni, but Andre-Pierre Gignac is benched in favour of the Ayew brothers.
Napoli: Reina; Maggio, Albiol, Fernandez, Armero; Inler, Dzemaili; Callejon, Pandev, Mertens; Higuain
Napoli bench: Rafael, Uvini, Cannavaro, Behrami, Hamsik, Insigne, Zapata
Marseille: Mandanda; Abdallah, N’Koulou, Diawara, Morel; Romao, Cheyrou; Thauvin, Valbuena, J Ayew; A Ayew
Marseille bench: Samba, Lemina, Imbula, Khalifa, Payet, B Mendy, Gignac