Fiorentina look to Alessandro Matri against Alberto Gilardino’s Genoa to close the gap on third-placed Napoli.
It kicks off at the Stadio Artemio Franchi at 19.45 GMT, click here for a match preview.
The Viola are in splendid form and a victory would take them within one point of Napoli in the last available Champions League spot.
They would also break away from Inter in fifth following the Nerazzurri’s 0-0 draw with Catania this afternoon.
Vincenzo Montella has a lengthy injury list to contend with, including Giuseppe Rossi, Mario Gomez, Nenad Tomovic and Borja Valero.
Gonzalo Rodriguez is also suspended, so there has to be a reshuffle at the back.
Matri scored a brace on his debut after joining from Milan, but immediately picked up a calf problem and is back in the starting XI tonight.
He is supported by Juan Guillermo Cuadrado and Joaquin, leaving ex-Genoa flop Juan Manuel Vargas on the bench.
Anderson arrived this week from Manchester United and is on the bench with his 'Andow' jersey.
Genoa’s star striker Gilardino wore the Fiorentina jersey for four years, scoring 48 Serie A goals in 118 games.
Alessandro Gamberini and Juraj Kucka are ruled out for Gian Piero Gasperini’s men, who beat Inter last week.
New signings Nicolas Burdisso and Paolo De Ceglie are thrown straight into the starting XI with Marco Motta on the bench.
Genoa’s last Serie A victory at the Stadio Franchi was back in March 1977 and they have lost their most recent seven here on the trot.
Fiorentina: Neto; Roncaglia, Compper, Savic; Pasqual, Pizarro, Aquilani, Ambrosini, Cuadrado; Joaquin, Matri
Fiorentina bench: Lupatelli, Rosati, Bakic, Rebic, Mati Fernandez, Woslki, Matos, Venuti, Vargas, Ilicic, Iakovenko, Olivera, Anderson
Genoa: Perin; Antonini, De Maio, Burdisso; Antonelli, Marchese, Matuzalem, De Ceglie; Konate, Bertolacci; Gilardino
Genoa bench: Donnarumma, Bizzarri, Stoian, Cofie, Calaio', Fetfatzidis, Vrsaljko, Motta, Centurion, Sturaro, Tavares, Portanova
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's World Cup tipster competition - £10,000 to be won!