Fiorentina must win to avoid sending Udinese through to their first Coppa Italia Final in 92 years.
It kicks off at the Stadio Artemio Franchi at 20.00 GMT and is the second leg of the semi-final.
The first leg ended 2-1 for Udinese, so a 1-0 home result would be enough to give the Viola victory on away goals.
Borja Valero is suspended in midfield, while Mario Gomez is back on the bench for the first time since damaging knee ligaments in September.
Juan Cuadrado, Alessandro Matri and Joaquin pour forward for the hosts.
Massimo Ambrosini is also sidelined and Gonzalo Rodriguez starts despite carrying a knock.
Vincenzo Montella’s men ended a run of one point from three games in all competition when beating Atalanta on Saturday.
Fiorentina haven’t reached the Coppa Italia Final since their triumph in 2001 with Roberto Mancini on the bench.
Udinese have never won the trophy and only reached the Final once in its debut year, 1922.
Simply getting into the Final could be enough to qualify them for Europe next season, as both Roma and Napoli in the other semi-final look set to book their spots via Serie A position.
The Friulani rely on veteran Antonio Di Natale supported by Bruno Fernandes and Roberto Pereyra, as goalscorer Luis Muriel is again starting from the bench.
Silvan Widmer is one to watch in midfield and once again 17-year-old Simone Scuffet stands in for Zeljko Brkic.
Udinese have already knocked Inter and Milan out of the competition this season. However, their Coppa record in Florence is of four consecutive defeats in which they scored one and conceded 10.
Fiorentina: Neto; Diakite, Rodriguez, Savic, Pasqual, Pizarro, Aquilani, Mati Fernandez; Cuadrado, Joaquin, Matri
Fiorentina bench: Rosati, Lupatelli, Roncaglia, Compper, Bakic, Vargas, Anderson, Matos, Wolski, Ilicic, Gomez
Udinese: Scuffet; Heurtaux, Danilo, Domizzi; Widmer, Pinzi, Allan, Gabriel Silva; Fernandes, Pereyra; Di Natale
Udinese bench: Kelava, Naldo, Bubnjic, Basta, Jadson, Yebda, Badu, Lazzari, Nico Lopez, Zielinski, Maicosuel, Muriel