Roma protested the “ambiguous and confusing” rules after a two-match crowd ban for anti-Neapolitan chants.
The Giallorossi will have to play their next two home games with the Curvas closed, as on Friday their appeal was rejected.
“We are very disappointed with the Court's decision. We presented concrete motions which were rejected,” read a statement on the Roma website.
“We remain steadfastly against all forms of discrimination. The current regulations, however, are ambiguous and their application to date has served to create confusion rather than address the real problems our sport faces.”
The anti-racism rules have almost exclusively been used to penalise ‘territorial discrimination’ – insulting chants or banners aimed at Neapolitans.
The ultras argue this is not what the regulations were designed for and are trying to outlaw decades-old local rivalry.
Clubs also protest that the bans damage the majority of fans rather than those few who are responsible for the chants.
The Disciplinary Commission already adjusted its approach once this season, going from ordering an entire game behind closed doors to merely shutting down the area of the stadium where offensive chants originated from.
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's World Cup tipster competition - £10,000 to be won!