Gonzalo Rodriguez, Juan Cuadrado and Juan Vargas are out of Fiorentina’s Europa League trip to Denmark against Esbjerg.
The first leg of the Round of 32 tie kicks off on Thursday at 18.00 GMT.
Rodriguez and Cuadrado are not included after they limped off during Saturday’s 2-1 home defeat to Inter, while Vargas is sidelined with a fractured bone in his hand.
Mario Gomez does make the trip to Denmark following his comeback as a substitute at the weekend, his first appearance since undergoing knee surgery in September.
The Viola squad will set out for Denmark on Wednesday morning.
Fiorentina squad for Esbjerg: Ambrosini, Aquilani, Borja Valero, Bakic, Bardini, Compper, Empereur, Fazzi, Fernandez, Gomez, Ilicic, Joaquin, Matri, Neto, Pasqual, Pizarro, Roncaglia, Rosati, Ryder, Savic, Tomovic