Borja Valero sits out a ban as Fiorentina visit Juventus in the first of three meetings over two weeks.
The Serie A encounter kicks off on Sunday at 11.30 GMT, click here for a match preview.
Borja Valero is suspended after his four-match ban was reduced to three on appeal, though he continues to maintain he did not push or insult the referee.
Nenad Tomovic is also ruled out by the Disciplinary Commission with Giuseppe Rossi and Ante Rebic sidelined.
These two teams will go head-to-head in the Europa League Round of 16 on March 13 and 20.
Fiorentina squad for Juventus: Ambrosini, Anderson, Aquilani, Bakic, Compper, Cuadrado, Diakite, Mati Fernandez, Gomez, Ilicic, Joaquin, Lupatelli, Matri, Neto, Pasqual, Pizarro, Rodriguez, Roncaglia, Rosati, Matos Ryder, Savic, Vargas, Wolski