Angelo Ogbonna and Andrea Barzagli are out of the Europa League Fiorentina-Juventus showdown tomorrow.
The first leg ended 1-1 in Turin and the decider kicks off on Thursday at 18.00 GMT.
Barzagli and Federico Peluso have been ruled out for several weeks with muscular problems, but they are now joined by Ogbonna.
He did not take part in training this morning and the club confirmed tests “revealed a first grade tear of his adductor longus muscle.”
This is effectively a thigh strain and will keep the defender out for a couple of weeks.
It leaves the Bianconeri short-staffed at the back for this game and the upcoming Serie A tests too.
Carlos Tevez and Mirko Vucinic are available again, but Antonio Conte has called up two youth team players.
Juventus squad for Fiorentina: Buffon, Chiellini, Caceres, Pogba, Vucinic, Tevez, Llorente, Osvaldo, Bonucci, Padoin, Pirlo, Asamoah, Vidal, Lichtsteiner, Storari, Isla, Rubinho, Mattiello, Romagna