Inter defender Rolando and Lazio benchwarmer Helder Postiga are in Portugal’s provisional World Cup squad.
Coach Paulo Bento will cut the side down to a final 23 by June 2.
Some of those called up have suffered from several injury problems, including Postiga, who barely featured since joining Lazio on loan in January.
Ricardo Quaresma is called up two years after his last international appearance.
Portugal will face Germany, Ghana and the USA in Group G.
Portugal World Cup squad
Goalkeepers: Anthony Lopes (Lyon), Beto (Sevilla), Eduardo (Braga), Rui Patricio (Sporting)
Defenders: Andre Almeida (Benfica), Antunes (Malaga), Bruno Alves (Fenerbahce), Fabio Coentreo (Real Madrid), Joao Pereira (Valencia), Neto (Zenit), Pepe (Real Madrid), Ricardo Costa (Valencia), Rolando (Inter)
Midfielders: Andre Gomes (Benfica), Joao Mario (Vitoria), Joao Moutinho (Monaco), Miguel Veloso (Dinamo Kiev), Raul Meireles (Fenerbahce), Ruben Amorim (Benfica), William Carvalho (Sporting)
Forwards: Cristiano Ronaldo (Real Madrid), Eder (SC Braga), Helder Postiga (Lazio), Hugo Almeida (Besiktas), Ivan Cavaleiro (Benfica), Nani (Manchester United), Rafa (Braga), Ricardo Quaresma (FC Porto), Varela (FC Porto), Vieirinha (Wolfsburg)
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's tipster competition - £11,000 to be won monthly!