Claudio Lotito asked fans not to “abandon” Lazio after disastrous season ticket sales this summer.
The ultras have long waged war on their President, boycotting games and official merchandise in protest at the way he runs the club.
That has been reflected in season ticket sales, which have reportedly been very poor so far this summer.
“I am well aware of the obsessive presence of some who want to mystify reality, who for many years have been holding me hostage by playing against the interests of the club,” wrote Lotito in an open letter.
“But my effort is based exclusively on reinforcing the squad and achieving results, certainly not to seek controversy.
“Lazio have installed a model of participation aimed not only at single fans, but to entire families, giving special discounts so every father can bring his children to the stadium to cheer on the Biancocelesti.
“I have already expressed my confusion at this boycott and repeat my appeal to the fans in the Curva Nord, Curva Sud, Distinti, Tevere and Monte Mario sections.
“I ask them again not to abandon Lazio. I ask them to stay united with the jersey and begin again for a new season of success.”