Italy rely on Domenico Berardi against Harry Kane’s England, but even a victory might not be enough to continue the European Under-21 Championship.
It kicks off at 19.45 UK time (18.45 GMT) at the Ander Stadium in Olomouc.
This concludes Group B and only two teams can go through to the semi-finals of the tournament.
The Azzurrini have only one point and need a victory, but also for Portugal to beat Sweden.
England can qualify with a win, or even a draw if Sweden fail to beat Portugal in the other game.
Stefano Sturaro is still suspended following his reaction foul in the opening match against Sweden.
Coach Gigi Di Biagio confirmed Berardi is “not 100 per cent fit,” but the Sassuolo striker starts anyway, as he is the leading light of this team.
Marcello Trotta of Avellino steps in to complete the trident with Palermo man Andrea Belotti.
England’s big star is Kane, but the Tottenham forward has struggled to make any kind of impact so far in the tournament.
John Stones returns after suffering from concussion, though Said Berahino and Alex Pritchard have been ruled out of the competition.
The record between these sides at Under-21 level is five Italy wins, four for England and three stalemates.
They last met two years ago in this very tournament, the Azzurrini making their way to the Final.
England U21: Butland; Jenkinson, Stones, Gibson, Garbutt; Chalobah, Forster-Caskey; Redmond, Ings, Lingard; Kane
England bench: Bond, Bettinelli, Keane, Moore, Chambers, Targett, Pritchard, Ward-Prowse, Carroll, Hughes, Loftus-Cheek, Afobe
Italy U21: Bardi; Zappacosta, Rugani, Romagnoli, Biraghi; Benassi, Crisetig, Cataldi; Berardi, Trotta, Belotti
Italy bench: Sportiello, Leali, Sabelli, Barba, Bianchetti, Izzo, Viviani, Baselli, Battocchio, Bernardeschi, Verdi