Roma start Edin Dzeko, Mohamed Salah and Lucas Digne against reigning Champions League holders Barcelona with Leo Messi, Neymar and Luis Suarez.
It kicks off at 19.45 UK time (18.45 GMT), follow all the action as it happens on our LIVEBLOG.
Luis Enrique returns to the Stadio Olimpico after his unsuccessful stint as Coach in 2011-12, but insists he has only “positive memories” of the experience.
Roma start Edin Dzeko, Mohamed Salah and Lucas Digne against reigning Champions League holders Barcelona with Leo Messi, Neymar and Luis Suarez.
It kicks off at 19.45 UK time (18.45 GMT), follow all the action as it happens on our LIVEBLOG.
Luis Enrique returns to the Stadio Olimpico after his unsuccessful stint as Coach in 2011-12, but insists he has only “positive memories” of the experience.
He’s since gone on to win the Treble at Barcelona and added the European Super Cup to that haul this summer.
These sides met in a pre-season friendly on August 5 at Camp Nou, with Barcelona winning 3-0, but that had many of the Giallorossi’s star names missing.
The Olimpico hasn’t always been kind to Roma, as last season they lost three out of five home fixtures in European competition.
Most notably, that included a 7-1 result to Bayern Munich.
Miralem Pjanic is still out with a calf injury, so Radja Nainggolan, Daniele De Rossi and Seydou Keita start in midfield.
Salah and Dzeko are joined by Iago Falque, who was in the Barcelona youth academy.
Barcelona are without Dani Alves, Thomas Vermaelen, Douglas and Bravo, so Jeremy Mathieu moves into central defence rather than Javier Mascherano.
The MSN trio of Neymar, Messi and Suarez are all present and correct.
Roma: Szczesny; Florenzi, Rudiger, Manolas, Digne; Nainggolan, De Rossi, Keita; Salah, Dzeko, Falque
Roma bench: De Sanctis, Maicon, Torosidis, Iturbe, Vainqueur, Totti, Gervinho
Barcelona: Ter Stegen; Sergi Roberto, Piqué, Mathieu, Jordi Alba; Iniesta, Busquets, Rakitic; Neymar, Messi, Suarez
Barcelona bench: Masip, Mascherano, Bartra, Adriano, Rafinha, Munir El Haddadi, Sandro Ramirez