Napoli are the only Europa League team not to change their squad, while Lazio confirm new signing Milan Bisevac.
Fiorentina, Serie A’s other representative in the competition, revamped their squad on Tuesday after making a handful of January signings.
However, Napoli haven’t added new arrival Vasco Regini and fellow signing Alberto Grassi has only been named in their B list.
Lazio have only made one change to their squad, adding Bisevac and removing the injured Stefan De Vrij.
The Aquile visit Galatasaray in the round of 32 on 16 February, while Fiorentina host Tottenham Hotspur and Napoli travel to Villarreal.