Felipe Anderson and Mauricio Pinilla start as Lazio and Genoa go head-to-head for a place in the Coppa Italia quarter-finals.
It kicks off at the Stadio Olimpico at 20.00 GMT and the winners go on to face Inter.
Simone Inzaghi’s Lazio have been impressive so far this season in Serie A and want to continue their fine form ahead of this weekend’s showdown with Juventus.
Keita Balde Diao is at the Africa Cup of Nations, while Luis Alberto and Dusan Basta are on the treatment table.
Felipe Anderson starts with Filip Djordjevic and Cristiano Lombardi in a 4-3-3 formation.
Genoa need to recover from a series of disappointing Serie A results, but Mattia Perin, Miguel Veloso and Davide Biraschi miss out.
New signing Adel Taarabt isn’t fully fit yet, but Pinilla does get to start with Lucas Ocampos and Luca Rigoni, as Giovanni Simeone has flu.
There are reports Lazio asked Genoa to drop Danilo Cataldi, the midfielder they loaned out last week, for this head-to-head match.
In Italy there are no rules barring loan players from taking on their parent clubs, so this would be a highly-unusual move.
Lazio: Strakosha; Patric, Wallace, Hoedt, Lukaku; Parolo, Biglia, Lulic; Lombardi, Djordjevic, Felipe Anderson
Lazio bench: Marchetti, Vargic, Bastos, De Vrij, Radu, Leitner, Milinkovic-Savic, Murgia, Kishna, Immobile, Rossi
Genoa: Lamanna; Munoz, Burdisso, Orban; Lazovic, Cofie, Rigoni, Laxalt; Pandev, Ocampos; Pinilla
Genoa bench: Rubinho, Zima, Brivio, Gentiletti, Izzo, Edenilson, Morosini, Ntcham, Beghetto, Taarabt, Ninkovic