Juventus have changed system again for a 4-3-3, while Fiorentina focus on Juan Manuel Vargas and Stevan Jovetic.
The midweek round kicks off this evening at 19.45 UK time at the Juventus Stadium in Turin with one of the big grudge matches of Serie A football.
Gianluigi Buffon and Emanuele Giaccherini are still out injured, but Arturo Vidal has returned from his time on the treatment table.
Vidal is thrown into the line-up in a midfield role for the new 4-3-3 system, as Mirko Vucinic and Simone Pepe flank Alessandro Matri.
Fiorentina are in crisis at the moment with fans calling for Coach Sinisa Mihajlovic to be sacked.
He'll have to do without Riccardo Montolivo, who has picked up a one-match ban following his yellow card in the last round, while Mattia Cassani and Per Kroldrup are sidelined.
It was thought Alberto Gilardino might return to the starting XI much sooner than expected after he was originally ruled out for up to two months with a sprained knee, but he is on the bench.
Instead Vargas and Alessio Cerci support Jovetic in the Viola front three.
Juventus: Storari; Lichtsteiner, Barzagli, Bonucci, Chiellini; Marchisio, Pirlo, Vidal; Pepe, Matri, Vucinic
Juventus bench: Manninger, Pazienza, Del Piero, De Ceglie, Elia, Quagliarella, Estigarribia
Fiorentina: Boruc; De Silvestri, Gamberini, Natali, Pasqual; Munari, Behrami, Kharja; Cerci, Jovetic, Vargas
Fiorentina bench: Neto, Silva, Gilardino, Nastasic, Lazzari, Ljajic, Romulo