Tommaso Rocchi is favoured to Mauro Zarate, while Mirko Vucinic, Marco Borriello and Jeremy Menez start together in the Derby della Capitale.
The local clash is set to kick off at the Olimpico at 14.00 UK time this afternoon.
Serie A leaders Lazio have opted for veteran Rocchi to partner Sergio Floccari and Hernanes, leaving Zarate surprisingly on the bench.
Nando Muslera is able to start after shaking off his injury concerns.
Roma are without captain Francesco Totti due to a one-match ban, while Juan and Rodrigo Taddei are sidelined.
David Pizarro had returned to the squad after his injury, but is only fit for the bench, so Fabio Simplicio starts in midfield.
Menez acts as trequartista behind Borriello and Vucinic. There were also doubts on Nicolas Andre Burdisso, but he is in the starting XI.
Lazio: Muslera; Lichtsteiner, Stendardo, Dias, Radu; Brocchi, Ledesma, Mauri; Hernanes; Rocchi, Floccari
Lazio bench: 12 Berni, 5 Scaloni, 14 Garrido, 11 Matuzalem, 23 Bresciano, 17 Foggia, 10 Zarate
Roma: Julio Sergio; Cassetti, Mexes, N Burdisso, Riise; Perrotta, De Rossi, Simplicio; Menez; Borriello, Vucinic
Roma bench: 1 Lobont, 25 G Burdisso, 2 Cicinho, 3 Castellini, 7 Pizarro, 89 Okaka, 8 Adriano
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's tipster competition - £11,000 to be won monthly!