Robbie Keane, John O'Shea and Aiden McGeady are out of the Republic of Ireland's friendly with Italy on Tuesday.
Giovanni Trapattoni's men will take on the Azzurri in an international test in Belgium tomorrow evening.
Following a 2-0 European Championship qualifying win over Macedonia at the weekend, Ireland are missing several stars.
Ex-Inter striker Keane has pulled out with a groin problem and Keith Fahey has a knee injury.
Shay Given and Kevin Kilbane are also absent, as they were excused to take part in a coaching course.
O'Shea was released so he could be with his wife, who is due to give birth this week, while McGeady was recalled by Spartak Moscow.
"McGeady has been given permission by Trapattoni to leave the squad after his club were in contact to request if he could be released ahead of forthcoming club games," confirmed a Football Association of Ireland spokesman on Sky Sports.
It means Shane Long, Andy Keogh and Sean St Ledger are set to start against the Azzurri.