Leonel Galeano's move to Catania failed to beat the deadline, but Udinese, Siena, Cesena, Genoa, Fiorentina and Novara completed transfers.
Catania had a deal in place to sign Galeano on loan from Argentine giants Independiente. However, red tape held up the paperwork from South America and the 18.00 UK time deadline blocked the move.
Serie A clubs are now barred from signing any more players, but are still able to sell to an overseas side.
This is why players like Amauri, Vincenzo Iaquinta and Marco Borriello could still leave Juventus and Roma to go abroad.
Udinese loaned 23-year-old midfielder Mahamadou Sissoko to Scottish outfit Kilmarnock and Under-19 Czech international forward Matej Vydra to Club Brugge.
The Friulani have been very active on deadline day, signing Paraguayan striker Miguel Maria Medina Miranda, commonly known as Medina, on a permanent basis from Sport Colombia.
Siena snapped up Boca Juniors attacking midfielder Joel Acosta from Boca Juniors, though he had already been on trial with the Tuscans.
Cesena took winger Abdel Ghezzal on loan from Serie B side Bari.
Genoa loaned Gergely Rudolf to Panathinaikos, as he spent the last six months at Bari.
Fiorentina signed 17-year-old Ghanaian defender Nii Nortey Ashong from Triestina on loan with the option to buy.
Novara picked up four young players today. Alberto Bellino was bought from Juventus, while the Serie A newcomers took Matteo Vittone (Borgosesia), Ahmed Ghazi (Pavia) and Stefano Truzzi (Sampdoria) on loan.