Giuseppe Rossi is a doubt for Italy's meaningless Euro 2012 qualifier against Serbia on Friday because of injury.
The Villarreal striker scored twice in a practice match on Tuesday, but ended the session with a left knee problem that has troubled him in recent weeks.
The forward becomes boss Cesare Prandelli's latest injury concern for the upcoming ties versus Serbia and Northern Ireland.
Inter centre-forward Giampaolo Pazzini is expected to miss this week's game, while Federico Balzaretti has to prove his fitness.
Zenit left-back Domenico Criscito left the Azzurri's training base earlier in the day after a groin problem saw his enforced withdrawal from the set-up.
Italy, who have already qualified for the European Championship, took on a Lega Pro Under-20 side this afternoon in a game which ended 5-0 to Prandelli's boys.
As well as Rossi, Parma's Sebastian Giovinco grabbed a brace and Manchester City ace Mario Balotelli scored the other.