Miralem Pjanic is Roma's trequartista behind Bojan Krkic and Pablo Osvaldo, while Miroslav Klose and Djibril Cisse start for Lazio.
The Derby della Capitale is the big game of the weekend and the Stadio Olimpico is packed out for the 19.45 UK time kick-off.
The Giallorossi were without captain Francesco Totti, Juan, Rodrigo Taddei, Cicinho, Stefano Okaka and Bogdan Lobont.
In Totti's absence, Luis Enrique moves Miralem Pjanic up to the trequartista role behind Bojan Krkic and Pablo Daniel Osvaldo.
Gabriel Heinze and Simon Kjaer form a new central defensive partnership, while Fernando Gago replaces David Pizarro in midfield.
Maarten Stekelenburg returns to the side for the first time since he was accidentally knocked unconscious by Inter defender Lucio a month ago.
There were concerns in the Lazio camp when Klose returned early from international duty, but he has recovered from that knock.
Therefore the German international starts with Djibril Cisse and Brazilian Hernanes, while Stefano Mauri also returns from an injury lay-off, but is only on the bench.
There were security fears ahead of the game, as on Saturday the anti-capitalism protests in Rome escalated into riots with at least 12 arrests and 70 injured.
However, the situation in and around the Stadio Olimpico is very calm on the night of the match.
Lazio: Marchetti; Konko, Biava, Dias, Radu; Gonzalez, Ledesma, Brocchi; Hernanes; Cisse, Klose
Roma: Stekelenburg; Rosi, Heinze, Kjaer, Josè Angel; Gago, De Rossi, Perrotta; Pjanic; Bojan, Osvaldo