Andrea Barzagli could potentially be ruled out of the 2012 European Championship after the Italian picked up an injury.
The Juventus stopper has suffered a calf strain that Azzurri medics fear could see him sidelined for the next 20 days.
Italy are set to assess his condition before making a decision on whether to replace him in the squad with another stopper. They will have until 24 hours before their Spanish tie on Sunday to request a squad change.
Inter’s Andrea Ranocchia would be in pole position to be called up as he was in the 25-man squad before Cesare Prandelli reduced it to 23.
Reports today suggested that Italy could try out a Juventus three-man defence consisting of Barzagli, Leonardo Bonucci and Giorgio Chiellini at Euro 2012. A 3-5-2 now seems unlikely.
Italy lost 3-0 to Russia on Friday. Their preparations for the tournament have already been hampered by the match fixing investigation and an earthquake which saw their Luxembourg friendly cancelled.