Ruben Olivera replaces David Pizarro, as Fiorentina welcome a Lazio side with Miroslav Klose leading the way.
This intriguing fixture kicks off at the Stadio Franchi at 14.00 GMT and is a battle for places in Europe.
Lazio need a boost after they threw away a lead to be held 1-1 by Panathinaikos on Thursday, but miss Ederson, Marius Stankevicius, Federico Marchetti and the suspended Giuseppe Sculli.
Klose and Stefano Mauri passed fitness tests to take their place in the starting XI.
Fiorentina have Pizarro and Facundo Roncaglia banned, while Michele Camporese is on the treatment table.
Olivera is chosen to stand in for Pizarro in midfield, while Adem Ljajic partners Stevan Jovetic.
Fiorentina:Viviano; Savic, Rodriguez, Tomovic; Cuadrado, Fernandez, Olivera, Borja Valero, Pasqual; Ljajic, Jovetic
Fiorentina bench: Neto, Hegazi, El Hamdaoui, Aquilani, Lupatelli, Cassani, Seferovic, Della Rocca, Llama, Migliaccio, Toni, Romulo
Lazio: Bizzarri; Konko, Biava, Dias, Lulic; Candreva, Gonzalez, Ledesma, Hernanes, Mauri; Klose
Lazio bench: Carrizzo, Straicosha, Ciani, Scaloni, Rocchi, Kozak, Onazi, Radu, Cana, Brocchi, Cavanda, Floccari