Delio Rossi picks his first line-up for Sampdoria with Andrea Poli in a trequartista role as Lazio aim to continue their 10-game unbeaten run.
It has been a dramatic few days for Samp, who sacked Coach Ciro Ferrara to bring in ex-Lazio and Fiorentina boss Rossi on Monday.
The Blucerchiati have one point from three rounds and are sliding back towards the drop zone, though the dismissal still came as a surprise. Rossi began by facing his old club, who were pushing to stay as Scudetto dark horses.
Fernando Tissone sits out a ban for the home side along with the injured Maxi Lopez, but Poli takes a new role behind the front two Eder and Mauro Icardi.
Lazio miss Ederson, Lorik Cana, Eddy Onazi, Tommaso Rocchi and the suspended Antonio Candreva. Mauro Zarate is again dropped after a dispute.
Miroslav Klose leads the 4-5-1 formation with Andre Dias returning in defence.
History does not smile on the Aquile at Marassi, as they have managed only one point from their last six visits.
Sampdoria: Romero; Berardi, Rossini, Gastaldello, Costa; Krsticic, Obiang, Munari; Poli; Eder, Icardi
Sampdoria bench: Da Costa, Berni, De Silvestri, Castellini, Soriano, Renan, Pozzi, Poulsen, Austoni, Estigarribia, Juan Antonio
Lazio: Marchetti; Konko, Biava, Dias, Radu; Gonzalez, Hernanes, Ledesma, Mauri, Lulic; Klose
Lazio bench: Bizzarri, Carrizo, Scaloni, Dias, Cavanda, Diakite, Cana, Onazi, Floccari, Brocchi, Zarate, Kozak
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's World Cup tipster competition  - £10,000 to be won!