Dejan Stankovic, Lucio and MacDonald Mariga have not recovered in time to take on Juventus.
Inter have released the squad list for tomorrow night's trip to Turin and there are several important absentees.
It was hoped Stankovic or Lucio would be back in the side after long injury lay-offs, but they have been left behind.
Also missing out are the injured Diego Milito, Walter Samuel and suspended Cristian Chivu, who continues his four-match ban for punching Bari defender Marco Rossi.
Inter squad: Julio Cesar, Castellazzi, Orlandoni; Cordoba, Zanetti, Maicon, Ranocchia, Materazzi, Nagatomo, Natalino; Thiago Motta, Sneijder, Kharja, Cambiasso, Obi, Coutinho; Pazzini, Eto'o, Pandev, Alibec