Fabio Borini, Marco Verratti and Lorenzo Insigne face Isco, David De Gea and Alvaro Morata in the European Under-21 Championship Final.
It kicks off at the Teddy Stadium in Jerusalem at 17.00 UK time (16.00 GMT).
Italy are the most successful side in the history of the tournament, winning it five times, while Spain are the holders from 2011.
Devis Mangia’s boys in blue reached the Final unbeaten, dropping points only in a 1-1 draw with Norway after having secured qualification.
The Rojita run by Julen Lopetegui have won every game so far, scoring eight goals and not conceding any. They are unbeaten in 25 competitive matches, which includes 23 victories.
Morata is the top scorer in the tournament so far with four goals, even though he has only started one of the four ties, but he is given a spot in today’s XI.
The Azzurrini’s only absentee is Luca Marrone due to an injury he sustained in the opening match against England.
Borini is again picked to partner Ciro Immobile with Insigne on the left wing, leaving Manolo Gabbiadini on the bench.
The Liverpool and ex-Roma striker scored the decisive goal in the semi-final win over Holland.
Spain can count on Manchester United goalkeeper De Gea and Malaga starlet Isco, who have won praise during this competition in Israel.
Italy U21: Bardi; Donati, Bianchetti, Caldirola, Regini; Florenzi, Rossi, Verratti, Insigne; Immobile, Borini
Italy bench: Leali, Colombi, Biraghi, Capuano, Marrone, Bertolacci, Crimi, Gabbiadini, Destro, Sansone, Paloschi, Saponara
Spain U21: De Gea; Montoya, Bartra, Inigo Martinez, Moreno; Koke, Illarramendi, Thiago Alcantara; Tello, Morata, Isco
Spain bench: Marino, Joel, Nacho, Marc Muniesa, Alvaro, Carvajal, Camacho, Sarabia, Rodrigo, Muniain, Alvaro Vazquez
Ref: Jug (SVN)
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's World Cup tipster competition  - £10,000 to be won!