Fiorentina and Dnipro have already qualified, but this is a head-to-head for who’ll win the Europa League group.
It kicks off at the Stadio Artemio Franchi at 18.00 GMT, the final encounter in Group E with the Viola currently leading on 13 points and the Ukrainians on 12.
Simply avoiding defeat will be enough for Vincenzo Montella’s men to keep hold of the top spot and get a stronger draw in the Round of 32.
Fiorentina already beat Dnipro 2-1 away from home back in Matchday Two with Gonzalo Rodriguez and Massimo Ambrosini on target.
They are also looking to maintain their home run of five consecutive European victories at the Stadio Franchi.
Mario Gomez is still out injured, while Giuseppe Rossi is rested in favour of young Brazilian Ryder Matos, who is flanked by Joaquin and Juan Guillermo Cuadrado.
Dnipro’s attack is led by Roman Zozulya.
Fiorentina: Neto; Roncaglia, Rodriguez, Savic, Pasqual; Mati Fernandez, Ambrosini, Borja Valero; Cuadrado, Matos, Joaquin
Fiorentina bench: Munua, Compper, Tomovic, Pizarro, Aquilani, Ilicic, Rossi
Dnipro: Boyko; Fedetskiy, Mandzyuk, Cheberyachko, Vlad; Kankava, Rotan; Bruno Gama, Politylo, Konoplyanka; Zozulya
Dnipro bench: Vartsaba, Kravchenko, Kulakov, Kobakhidze, Blyznychenko, Kalinic, Seleznyov