Lazio have already qualified, but must beat Trabzonspor to win the Europa League group and end their crisis.
It kicks off at the Stadio Olimpico in Rome at 20.05 with both sides already sure of their spot in the Round of 32.
They drew 3-3 in Turkey when the Biancocelesti fought back from 3-1 down with two very late Sergio Floccari goals.
Vladimir Petkovic is feeling the pressure after a series of negative results in Serie A.
Miroslav Klose is back on the bench after a shoulder injury, while teenager Keita Balde is again thrown into the starting XI to support Floccari.
Hernanes is again benched in favour of Ederson and Cristian Ledesma, with Etrit Berisha in goal.
It is a happy occasion for Trabzonspor Coach Mustafa Akcay, who celebrated his 55th birthday today and will be hoping for a gift from his players.
Zokora sits out a ban, while Kadir Keles is injured, but Alanzinho, Florent Malouda and Paulo Henrique complete the attack.
Lazio: Berisha; Cavanda, Novaretti, Biava, Radu; Ledesma; Onazi, Felipe Anderson, Keita, Ederson; Floccari
Lazio bench: Marchetti, Ciani, Biglia, Hernanes, Lulic, Candreva, Klose
Trabzonspor: Kivrak; Bosingwa, Yumlu, Bamba, Demir; Colman, Aykut Adin; Olcan Adin, Malouda, Alanzinho; Paulo Henrique
Trabzonspor bench: Öztürk, Erdogan, Zeko Yavru, Mierzejewski, Abdulkadir Ozdemir, Emre Gural, Janko
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