Roma need a victory to keep up with Juventus and Napoli, but Sinisa Mihajlovic’s Sampdoria can cause an upset.
It kicks off at the Stadio Olimpico at 19.45 GMT, click here for a match preview .
It has been a tough week for the Giallorossi, who were held to a draw by rivals Lazio and went out of the Coppa Italia with a 3-0 semi-final defeat to Napoli on Wednesday.
The pressure is growing on Rudi Garcia’s men, as both Juve and Napoli won this afternoon.
There will be no fans in the Curva Sud or Curva Nord, as the club begins its two-match ban for anti-Neapolitan chants.
Francesco Totti is unavailable due to injury, joining Federico Balzaretti and Dodo in the stands.
Radja Nainggolan returns after his Coppa Italia ban, giving Daniele De Rossi a rest, but the trident attack this time consists of Alessandro Florenzi, Mattia Destro and Gervinho.
This is like a derby for Sampdoria Coach Mihajlovic, who confirmed he is a Lazio supporter after playing with the Biancocelesti for many years.
His team has improved markedly since he replaced Delio Rossi and are on a run of two consecutive victories, losing just once in the last five rounds.
Maxi Lopez is out with an injury, so Roberto Soriano and Wszolek complete the attack with Brazilian Eder and Galatasaray transfer target Manolo Gabbiadini.
Last season this fixture ended 1-1, but Samp have’t conquered the Olimpico since 2010. The teams also clashed in the Coppa Italia on January 9 with Roma squeezing past 1-0.
Roma: De Sanctis; Maicon, Benatia, Castan, Torosidis; Pjanic, Nainggolan, Strootman; Florenzi, Destro, Gervinho
Sampdoria: Da Costa; De Silvestri, Mustafi, Gastaldello, Regini; Palombo, Krsticic; Gabbiadini, Soriano, Wszolek; Eder
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's tipster competition  - £11,000 to be won monthly!