Fiorentina rely on Alberto Aquilani, Alessandro Matri and Borja Valero in their Europa League trip to Esbjerg.
It kicks off in Denmark at 18.00 GMT, the first leg in their Round of 32 tie.
The Viola are fresh from a surprise 2-1 home defeat to Inter on Saturday and are continuing to deal with injury worries.
Ruled out for this game are Juan Cuadrado, Juan Manuel Vargas, Gonzalo Rodriguez and Giuseppe Rossi.
Mario Gomez is on the bench after his comeback at the weekend, ending five months on the sidelines with a knee ligament injury.
Matri leads the way with Josip Ilicic and young Brazilian Ryder Matos in a 4-3-3 formation, while Borja Valero is in midfield after his Serie A ban.
David Pizarro and Joaquin are rested with Stefan Savic returning to defence following his injury.
Esbjerg progressed to this phase for the first time, having won four and lost two in the group stage.
Esbjerg: Dubravka; P Ankersen, Drobo-Ampem, Jakobsen, Knudsen; J Ankersen, Lekven, Andreasen, Lyng; Fellah, Pusic
Esbjerg bench: Hojberg, Bergvold, J Andersen, Lucena, Rasmussen, Van Buren, Bakenga
Fiorentina: Neto; Roncaglia, Savic, Compper, Pasqual; Aquilani, Mati Fernandez, Borja Valero; Ilicic, Matri, Matos Ryder
Fiorentina bench: Rosati, Tomovic, Pizarro, Bakic, Joaquin, Ambrosini, Gomez