Federico Bernardeschi is out of the Champions League tie between Juventus and Barcelona due to a muscular problem.
It kicks off on Wednesday at 19.45 GMT at the Allianz Juventus Stadium in Turin.
Stephan Lichtsteiner is not on the UEFA squad this season, with Marko Pjaca a long-term injury absentee.
Bernardeschi will be in the stands due to a muscular problem to his right thigh that he sustained in training.
Rodrigo Bentancur returns, having been left out of the 3-2 loss to Sampdoria on Saturday, while Gigi Buffon and Andrea Barzagli were rested.
Juventus squad for Barcelona: Buffon, De Sciglio, Chiellini, Benatia, Pjanic, Khedira, Cuadrado, Marchisio, Higuain, Dybala, Douglas Costa, Alex Sandro, Matuidi, Barzagli, Pinsoglio, Mandzukic, Howedes, Asamoah, Szczesny, Rugani, Sturaro, Bentancur