Mario Mandzukic and Paulo Dybala are passed fit to start, while Sergej Milinkovic-Savic and Ciro Immobile aim to give Lazio a third Juventus scalp.
It kicks off at 17.00 GMT, follow the build-up and action as it happens with the LIVEBLOG.
This is a huge fixture, as it’s third against second in Serie A and the Bianconeri can’t risk falling further behind leaders Napoli.
Both were in action on Wednesday for the Coppa Italia semi-final, but while Juve disposed of Atalanta 1-0 (2-0 on aggregate) thanks to a very debatable penalty, Lazio had to play 120 minutes and a sudden death penalty shoot-out, which they lost to Milan.
There is no time to recover, as they’re right back in action in a rainy Stadio Olimpico and seeking a third win of the season against Juve.
The Aquile beat them 3-2 in a thrilling Italian Super Cup clash in August, then ended Juve’s 57-match unbeaten home Serie A run.
Ciro Immobile is Capocannoniere and leads the attack with Luis Alberto and Sergej Milinkovic-Savic, but Martin Caceres is injured and Adam Marusic suspended with Dusan Basta not 100 per cent fit.
Jordan Lukaku made a big difference coming off the bench against Juve in the Super Cup, so he gets to start.
As Lazio play with a 3-5-2 formation, Max Allegri adjusts Juventus to mirror that system, with Giorgio Chiellini, Alex Sandro and Douglas Costa are rested ahead of the Champions League trip to Tottenham Hotspur.
Stephan Lichtsteiner and Kwadwo Asamoah take wide roles, with Dybala’s return to the starting XI for the first time in two months. The three-man defence only started once before in Serie A this season.
There were some concerns about Mandzukic, but the centre-forward has been passed fit to start despite a slight muscular issue in training.
It’s just as well, because Juve are missing Gonzalo Higuain, Juan Cuadrado, Federico Bernardeschi and Mattia De Sciglio.
The Bianconeri’s last Serie A defeat at the Olimpico was 2-0 in December 2013, followed by nine wins and three 1-1 draws.
Lazio: Strakosha; Luiz Felipe, De Vrij, Radu; Lulic, Parolo, Leiva, Milinkovic-Savic, Lukaku; Luis Alberto; Immobile
Lazio bench: Guerrieri, Vargic, Bastos, Basta, Patric, Murgia, Crecco, Jordao, Lukaku, Nani, Felipe Anderson, Caicedo
Juventus: Buffon; Barzagli, Rugani, Benatia; Lichtsteiner, Khedira, Pjanic, Matuidi, Asamoah; Dybala, Mandzukic
Juventus bench: Szczesny, Chiellini, Howedes, Sturaro, Douglas Costa, Bentancur, Marchisio, Del Favero, Alex Sandro
See the latest Serie A predictions and betting tips with Eurotips.co.uk