Ascoli will remain in Serie B and Virtus Entella are relegated after the play-out ended goalless.
Both the first and second leg of this relegation play-out finished 0-0.
In these circumstances, rather than go to extra time, the winners are the side that finished higher up the table during the regular season.
It means Ascoli and their Coach Serse Cosmi have done enough to stay in Serie B for another term.
Virtus Entella drop down to Serie C after four years, where they join Novara, Pro Vercelli and Ternana.