Sampdoria look to on-form Gregoire Defrel and Fabio Quagliarella against Fiorentina’s Giovanni Simeone and Federico Chiesa in the Week 1 fixture.
It kicks off at Marassi at 18.00 UK time (17.00 GMT).
This game was originally scheduled for August, but along with Milan-Genoa was postponed in the wake of the Ponte Morandi collapse, as the iconic Genoese bridge crumbled, killing 42 people.
Both these teams have ambitions of European football and have enjoyed very impressive results so far this season.
Samp thrashed Napoli 3-0 and put five past Frosinone.
Gianluca Caprari had been expected to miss out with a knock, but not only is he in the squad, he’s also starting behind Quagliarella and Defrel.
Fiorentina lost 1-0 to Napoli in a hard-fought game, ending their unbeaten record after two victories.
Marko Pjaca gets his first start in a Viola jersey, alongside Chiesa and Simeone, with Edimilson stepping in for Marco Benassi in midfield.
Sampdoria: Audero; Sala, Andersen, Tonelli, Murru; Barreto, Ekdal, Linetty; Caprari; Quagliarella, Defrel
Sampdoria bench: Rafael, Belec, Viera, Praet, Ramirez, Jankto, Colley, Bereszynski, Ferrari, Stijepovic, Rolando, Kownacki
Fiorentina: Dragowski; Milenkovic, Vitor Hugo, Pezzella, Biraghi; Edimilson, Veretout, Gerson; Pjaca, Simeone, Chiesa
Fiorentina bench: Lafont, Laurini, Ceccherini, Norgaard, Eysseric, Mirallas, Dabo, Hancko, Sottil, Benassi, Diks, Thereau