Roberto Mancini has still not decided who to use in attack against Portugal, as Ciro Immobile and Domenico Berardi are both tested.
The Nations League clash kicks off at San Siro on Saturday at 19.45 GMT.
Federico Bernardeschi is out injured, so the trident attack used against Poland has to change.
It had seemed as if Lazio forward Immobile would become the centre-forward, moving Lorenzo Insigne back to his more traditional wide left role.
However, in today’s training session, Sassuolo star Berardi was tested out with Insigne still acting as a False Nine.
The rest of the line-up remains identical to the one that won 1-0 in Poland last month.
That means Cagliari youngster Nicolò Barella resists competition from Roma’s Lorenzo Pellegrini in midfield.
Italy (probable): Donnarumma; Florenzi, Bonucci, Chiellini, Biraghi; Verratti, Jorginho, Barella; Chiesa, Immobile (Berardi), Insigne