Juventus are drawn Europa League holders Atletico Madrid in the Champions League last 16, while Roma take on Porto.
Juve last faced Atleti in the 2014-15 Champions League, when the Spaniards held them to a 0-0 draw and won 1-0 over two games to progress as group winners.
Diego Simeone's side also have European pedigree, losing two Finals to Real Madrid in 2014 and 2016, before winning the Europa League last season.
Juventus are drawn Europa League holders Atletico Madrid in the Champions League last 16, while Roma take on Porto.
Juve last faced Atleti in the 2014-15 Champions League, when the Spaniards held them to a 0-0 draw and won 1-0 over two games to progress as group winners.
Diego Simeone's side also have European pedigree, losing two Finals to Real Madrid in 2014 and 2016, before winning the Europa League last season.
Roma, meanwhile, have scores to settle against Porto, who triumphed 4-1 on aggregate to deny the Giallorossi access to the 16-17 Champions League group stage.
Elsewhere, Paul Pogba's Manchester United face Paris Saint-Germain and Liverpool lock horns with fellow giants Bayern Munich.
The first legs will be played across February and 12-13 and 19-20, with the return games scheduled for March 5-6 and 12-13.
Champions League last-16 draw in full:
Schalke v Manchester City
Atletico Madrid v Juventus
Manchester United v Paris Saint-Germain
Tottenham Hotspur v Borussia Dortmund
Olympique Lyonnais v Barcelona
Roma v Porto
Ajax v Real Madrid
Liverpool v Bayern Munich