Napoli options are limited due to an injury crisis, while Fiorentina are missing two key players and might adjust their tactics.
It kicks off at the Stadio Franchi at 17.00 GMT, click here for a match preview.
The Partenopei begin life after Marek Hamsik, as their captain is not even in the squad ahead of his €20m move to Dalian Yifang.
Defender Raul Albiol is injured and in doubt for the Europa League clash with FC Zurich on Thursday evening.
Arkadiusz Milik is not 100 per cent fit and unlikely to start, so it seems as if Dries Mertens and Lorenzo Insigne have to be the attacking partners.
Fabian Ruiz takes over from Hamsik in midfield with Allan, while Nikola Maksimovic should step in at centre-back.
Fiorentina have issues of their own to deal with, as Marco Benassi and Nikola Milenkovic are suspended, with Vitor Hugo and Vincent Laurini injured.
That leaves few alternatives in defence, as David Hancko and Federico Ceccherini are expected to start.
Stefano Pioli could shift his tactics to a 4-3-2-1 Christmas Tree formation, with Gerson and Federico Chiesa supporting Luis Muriel.
Another alternative is to maintain the 4-3-3, with Chiesa, Muriel and Kevin Mirallas.
All signs point to Giovanni Simeone being left on the bench, at least to begin with.
Fiorentina (probable): Lafont; Ceccherini, Pezzella, Hancko, Biraghi; Gerson, Edimilson Fernandes, Veretout; Chiesa, Muriel, Mirallas
Napoli (probable): Meret; Hysaj, Maksimovic, Koulibaly, Mario Rui; Callejon, Allan, Fabian Ruiz, Zielinski; Insigne, Mertens