Juventus have a total reshuffle of their line-up with Moise Kean, Alex Sandro and Federico Bernardeschi upfront, while Udinese give Ben Wilmot his debut.
It kicks off in Turin at 19.30 GMT.
Max Allegri practically secured an eighth consecutive Scudetto with a 2-1 win away to Napoli last week, extending the lead at the top of the table to 16 points.
As the second leg of the Champions League Round of 16 with Atletico Madrid is coming up, and a 2-0 deficit has to be overturned, the Coach takes this opportunity to rest numerous top players.
Mario Mandzukic, Cristiano Ronaldo, Giorgio Chiellini, Leonardo Bonucci and Paulo Dybala are among those left on the bench.
Miralem Pjanic and Joao Cancelo are suspended, with Sami Khedira, Juan Cuadrado, Mattia De Sciglio and Douglas Costa on the treatment table.
Alex Sandro is pushed up into a trident attack with teenager Kean and Bernardeschi.
Udinese must do without Rolando Mandragora, who sits out a ban against his former club, while Valon Behrami, Antonin Barak, Emmanuel Badu, Samir and Marco D’Alessandro are injured.
The Friulani won their last two games on the bounce, but are still hovering above the relegation zone and any point here would be precious.
Argentina international Rodrigo De Paul is supported by Ignacio Pussetto, while goalkeeper Juan Musso also wants to celebrate his recent debut senior Argentina call-up.
The real surprise is 19-year-old Ben Wilmot in midfield, given his Serie A debut after a January transfer from Watford.
He has one cap for England at Under-20 level.
Juventus: Szczesny; Caceres, Barzagli, Rugani, Spinazzola; Bentancur, Emre Can, Matuidi; Bernardeschi, Kean, Alex Sandro
Juventus bench: Perin, Pinsoglio, Chiellini, Bonucci, Kastanos, Nicolussi Caviglia, Mavididi, Dybala, Mandzukic, Ronaldo
Udinese: Musso; De Maio, Troost-Ekong, Nuytinck; Ter Avest, Stryger-Larsen, Wilmot, Fofana, Zeegelaar; Pussetto, De Paul
Udinese bench: Perisan, Nicolas, Opoku, Okaka, Ingelsson, Micin, Lasagna, Sandro, Bocic