Fiorentina and Lazio face off for precious points in the race for Europe, but while Thomas Strakosha and Ciro Immobile recover, Alban Lafont does not.
It kicks off at the Stadio Franchi at 19.30 GMT.
These are two of the most entertaining teams in Serie A and go head-to-head for continental ambitions, while they are also both in the Coppa Italia semi-finals.
Fiorentina miss goalkeeper Lafont with a sprained ankle, so Pietro Terracciano gets his Viola debut after a January transfer from Empoli.
Marko Pjaca’s season is over, as the unlucky Juventus-owned winger tore his ACL in training this week.
Gerson joins Luis Muriel and Federico Chiesa in the trident attack, leaving Giovanni Simeone on the bench.
Lazio goalkeeper Strakosha had also been in doubt, but has recovered in time from a shoulder injury he sustained during the 3-0 Derby della Capitale victory over Roma.
Immobile and Senad Lulic also shake off fitness concerns to start this very important match.
This will be Stefan Radu's 342nd official appearance in a Lazio jersey, placing him joint fourth in the all-time club record books with Aldo Puccinelli of the 1940s.
He is now preceded only by Paolo Negro (376), Giuseppe Wilson (394) and Giuseppe Favalli (401).
Wallace and Jordan Lukaku are injured, but Patric is somewhat surprisingly used at centre-back.
Former Fiorentina captain Milan Badelj is on the bench in this first visit back to the Stadio Franchi.
This should be entertaining, because there have been 16 goals in the last three editions of Fiorentina-Lazio at the Stadio Franchi, an average of 5.3 per game.
Fiorentina: Terracciano; Ceccherini, Pezzella, Milenkovic, Biraghi; Benassi, Edimilson Fernandes, Veretout; Chiesa, Muriel, Gerson
Fiorentina bench: Lafont, Brancolini, Vitor Hugo, Hancko, Laurini, Dabo, Noorgard, Graiciar, Mirallas, Simeone
Lazio: Strakosha; Patric, Acerbi, Radu; Marusic, Milinkovic-Savic, Leiva, Luis Alberto, Lulic; Correa, Immobile
Lazio bench: Proto, Guerrieri, Bastos, Luiz Felipe, Durmisi, Romulo, Jordao, Cataldi, Badelj, Parolo, Neto, Caicedo