Juventus start Federico Bernardeschi and Leonardo Spinazzola, but drop Paulo Dybala, while Atletico Madrid start Alvaro Morata.
The second leg of the Champions League Round of 16 kicks off in Turin at 20.00 GMT, with the Bianconeri losing the first 2-0 in Spain.
It’s a huge task to overturn a 2-0 deficit and keep their Champions League dreams alive, especially as Max Allegri does not have a full squad to choose from.
Alex Sandro is suspended, with Douglas Costa, Mattia De Sciglio, Andrea Barzagli, Juan Cuadrado and Sami Khedira injured.
He had been tempted by the 3-5-2 formation with Martin Caceres at centre-back, but instead sticks with the usual 4-3-3, albeit with some adjustments.
Spinazzola makes his career Champions League debut at left-back, with Joao Cancelo on the right.
In attack, Bernardeschi gets the nod rather than Dybala, partnering Cristiano Ronaldo and Mario Mandzukic.
Emre Can is chosen in midfield, sending Rodrigo Bentancur to the bench.
Atletico Madrid also have some issues to deal with, as Diego Costa and Thomas Partey sit out bans, with Filipe Luis and Lucas Hernandez injured.
Diego Godin, who is set to join Inter next season as a free agent, has shaken off a knock to start in defence.
Santiago Arias at right-back role in place of Filipe Luis, despite reports over the last few hours that Saul Niguez would go at left-back instead.
During an injury crisis in December, Saul did play at left-back for seven games, but never on the right.
Vitolo is therefore benched in favour of Saul in his more traditional role, with Koke, Rodrigo and Lemar completing the midfield.
Alvaro Morata faces his former Juventus teammates, standing in for suspended Costa, alongside Atleti legend Antoine Griezmann.
The Colchoneros have kept five consecutive Champions League clean sheets, so are the toughest side with which to overturn a 2-0 first leg defeat.
To make matters worse, historically Juventus have never beaten Atletico Madrid or scored a goal against them, managing only a 0-0 draw here in December 2014 and two away defeats.
Juventus: Szczesny; Cancelo, Bonucci, Chiellini, Spinazzola; Can, Pjanic, Matuidi; Bernardeschi, Mandzukic; Ronaldo
Juventus bench: Perin, Caceres, Dybala, Kean, Rugani, Bentancur, Nicolussi Caviglia
Atletico Madrid: Oblak; Arias, Gimenez, Godin, Juanfran; Koke, Rodrigo, Saul, Lemar; Griezmann; Morata
Atletico Madrid bench: Adan, Savic, Montero, Vitolo, Mikel Carro, Kalinic, Correa
Ref: Kuipers (NED)