Fiorentina and Napoli face off with Dries Mertens and Lorenzo Insigne against a surprisingly youthful Viola line-up.
It kicks off at 19.45 UK time (18.45 GMT, 20.45 CET), follow all the build-up and action as it happens on the LIVEBLOG.
The Partenopei are hoping to end the Juventus Scudetto winning streak after eight years and visit the Stadio Franchi, where their title hopes were so famously destroyed two seasons ago.
Arkadiusz Milik is not 100 per cent fit and Hirving Lozano just arrived, so Mertens leads the attack with support from Jose Callejon, Fabian Ruiz and Insigne in a 4-2-3-1 formation.
Piotr Zielinski and Fabian Ruiz are capable of swapping places during the game.
The new faces are in defence, with ex-Roma centre-back Kostas Manolas and former Empoli talent Giovanni Di Lorenzo, but Mario Rui is preferred to Faouzi Ghoulam.
Fiorentina are revamped with new owner Rocco Commisso, who has flown in from New York to sit in the stands for tonight’s Serie A opener.
They are still short on strikers with Giovanni Simeone shoved aside and Boateng stuttering in the centre-forward role, so Vincenzo Montella picks 19-year-old Dusan Vlahovic to lead the line.
He is supported by Federico Chiesa and Boateng, moved to a wider position, while Franck Ribery is on the bench just days after his introduction.
Cristiano Biraghi is reported to be on the verge of a move to Inter, where he’d swap with Henrique Dalbert, so he is on the bench too.
It means Montella puts his faith in 24-year-old Lorenzo Venuti, 22-year-old Gaetano Castrovilli and 20-year-old Riccardo Sottil, son of former Fiorentina defender Andrea.
Fiorentina: Dragowski; Lirola, Milenkovic, Pezzella, Venuti; Castrovilli, Badelj, Pulgar; Chiesa, Vlahovic, Sottil
Fiorentina bench: Terraciano, Biraghi, Ranieri, Ribery, Simeone, Boateng, Cristoforo, Ceccherini, Montiel, Benassi, Zurkowski, Terzic
Napoli: Meret; Di Lorenzo, Manolas, Koulibaly, Mario Rui; Allan, Zielinski; Callejon, Fabian Ruiz, Insigne; Mertens
Napoli bench: Ospina, Karnezis, Malcuit, Verdi, Elmas, Luperto, Maksimovic, Chiriches, Hysaj, Ghoulam, Younes, Gaetano