Franck Ribery is tipped to get his first start in a Fiorentina jersey, while Juventus bring in Adrien Rabiot with Cristiano Ronaldo and Gonzalo Higuain.
It kicks off at the Stadio Artemio Franchi at 14.00 UK time (13.00 GMT), click here for a match preview.
The Viola are still looking for their first point of the season after defeats to Napoli and Genoa, but are without Marco Benassi and new signing Pedro Guilherme.
It means Ribery is expected to get his full Serie A debut, having been a late substitute in both previous games, alongside Kevin-Prince Boateng and Federico Chiesa.
Former Inter left-back Henrique Dalbert is also due to start with Erick Pulgar in midfield.
Juventus defend their 100 per cent record, but make the trip without Mario Mandzukic (who is in advanced talks with clubs from Qatar and MLS), Mattia De Sciglio, Giorgio Chiellini, Marko Pjaca and Mattia Perin.
Maurizio Sarri can finally take his place on the bench after missing the first two rounds with pneumonia.
He is not expected to make many changes, though, with Rabiot coming in for Blaise Matuidi the only real adjustment from the side that beat Napoli 4-3.
Matthijs de Ligt again stands in for injured captain Chiellini, while Douglas Costa, Gonzalo Higuain and Cristiano Ronaldo make up the trident attack.
Fiorentina (4-3-3): Dragowski; Lirola, Milenkovic, Pezzella, Dalbert; Pulgar, Badelj, Castrovilli; Chiesa, Boateng, Ribery
Juventus (4-3-3): Szczesny; Danilo, Bonucci, De Ligt, Alex Sandro; Khedira, Pjanic, Rabiot; Douglas Costa, Higuain, Ronaldo
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