Fiorentina rely on Federico Chiesa and Giovanni Simeone against age-old rivals Juventus, who start Cristiano Ronaldo, Paulo Dybala and Juan Cuadrado.
It kicks off at 17.00 GMT at the Stadio Franchi.
You can follow the build-up and action as it happens from this game and Sampdoria v Bologna on the LIVEBLOG.
The Viola have won only two of their last 10 matches and come into the back of this off five consecutive stalemates.
Fiorentina rely on Federico Chiesa and Giovanni Simeone against age-old rivals Juventus, who start Cristiano Ronaldo, Paulo Dybala and Juan Cuadrado.
It kicks off at 17.00 GMT at the Stadio Franchi.
The Viola have won only two of their last 10 matches and come into the back of this off five consecutive stalemates.
Only once before have they drawn six Serie A games in a row, back in 1989-90.
There is no fixture they’d like to win more than this one, as the rivalry with Juve goes back decades.
The Bianconeri are also unbeaten this season in Serie A, dropping points only in a 1-1 home draw with Genoa.
German Pezzella recovers from an injury to bolster the defence, while Juve-owned Marko Pjaca is only on the bench.
Chiesa, Simeone and Gerson complete the trident attack, with Jordan Veretout and Marco Benassi in midfield.
Juventus miss Alex Sandro, Sami Khedira and Emre Can, while Miralem Pjanic is rested in favour of Rodrigo Bentancur as the playmaker.
Cuadrado takes a more central midfield role than he is accustomed to against his former club.
Dybala, Mario Mandzukic and Cristiano Ronaldo form the now traditional front three.
Juve have won two of the last three trips to the Stadio Franchi, but Fiorentina are unbeaten at home this season, conceding only three goals.
Although there is rivalry, Juve players will lay flowers at the Viola bench before kick-off in honour of Davide Astori, their former Italy teammate.
Unfortunately, some still refuse to be civilised about the sport and graffiti appeared near the stadium referencing Heysel and Juve legend Gaetano Scirea.
Fiorentina: Lafont; Milenkovic, Pezzella, Hugo, Biraghi; Edimilson, Benassi, Veretout; Chiesa, Simeone, Gerson
Juventus: Szczesny; De Sciglio, Bonucci, Chiellini, Cancelo; Cuadrado, Bentancur, Matuidi; Dybala, Mandzukic, Cristiano Ronaldo
Ref: Orsato