Fiorentina use Federico Chiesa and Franck Ribery in a 3-5-2 formation, while Maurizio Sarri is on the Juventus bench, starting Cristiano Ronaldo and Douglas Costa.
It kicks off at the Stadio Artemio Franchi at 14.00 UK time (13.00 GMT).
You can follow all the build-up and action as it happens and give your views on today’s three Serie A games on the LIVEBLOG.
This fixture is famously a bitter grudge match and even more important to the Viola, seeing as they have lost both their opening games under Coach Vincenzo Montella.
In fact, Fiorentina are without a win in 16 Serie A rounds, so one off their worst run since 1970-71.
The Tuscans haven’t won their last 12 top flight home matches, already a club record.
Marco Benassi is carrying an injury and Pedro Guilherme not ready to play after his deadline day move from Fluminense, so Kevin-Prince Boateng was expected to start with Chiesa and Ribery in a 4-3-3 formation.
Instead, Montella has moved to a 3-5-2 with Martin Caceres starting against his former club, Chiesa and Ribery in more central purely attacking roles than they are perhaps accustomed to.
New signing Rachid Ghezzal is also on the bench waiting to make how bow.
Juventus had a perfect start to the campaign, albeit with narrow wins over Parma and Napoli, but the big change is that Sarri makes his first appearance on the bench after recovering from pneumonia.
It is fitting he does so in Florence, just a stone’s throw away from his hometown and against the team his family supports.
Giorgio Chiellini, Mattia De Sciglio, Marko Pjaca and Mattia Perin are injured, so Matthijs de Ligt again partners Leonardo Bonucci after some hiccups in the 4-3 thriller with Napoli.
Douglas Costa and Cristiano Ronaldo support Gonzalo Higuain, who is again chosen to lead the line, with Blaise Matuidi once more getting the midfield nod rather than Adrien Rabiot.
Pipita does enjoy facing Fiorentina, as in 11 Serie A meetings, he scored six and assisted three goals.
The Bianconeri have won their last two Serie A trips to Florence, but haven’t managed a hat-trick in this fixture since 1952.
Fiorentina: Dragowski; Milenkovic, Pezzella, Caceres; Lirola, Castrovilli, Pulgar, Badelj, Dalbert; Chiesa, Ribery
Fiorentina bench: Terracciano, Ranieri, Boateng, Sottil, Cristoforo, Ceccherini, Ghezzal, Montiel, Venuti, Zurkowski, Vlahovic, Terzic
Juventus: Szczesny; Danilo, De Ligt, Bonucci, Alex Sandro; Khedira, Pjanic, Matuidi; Douglas Costa, Higuain, Cristiano Ronaldo
Juventus bench: Pinsoglio, Buffon, Ramsey, Dybala, Cuadrado, Can, Rugani, Rabiot, Demiral, Bentancur, Bernardeschi
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