Gigi Buffon makes history, while Juan Cuadrado and Blaise Matuidi become full-backs as Juventus host SPAL and their surprise striker pick.
It kicks off in Turin at 14.00 UK time (13.00 GMT).
Follow all the build-up and action as it happens from this game, Sampdoria-Inter and Sassuolo-Atalanta on the LIVEBLOG.
The Bianconeri find themselves in an injury crisis, as Mattia De Sciglio, Douglas Costa, Danilo and Giorgio Chiellini are out of action.
Alex Sandro flew back to Brazil on compassionate leave this week following a death in the family.
Cuadrado has at least been training as a right-back for several weeks under Coach Maurizio Sarri, but there is a desperate shortage of left-backs.
The solution for this game is to use midfielder Matuidi on the left side of defence, introducing Adrien Rabiot in the middle of the park with Sami Khedira and Miralem Pjanic.
Cristiano Ronaldo returns after he was rested for Tuesday’s 2-1 win at Brescia, so Paulo Dybala starts rather than Gonzalo Higuain, and Aaron Ramsey retains his trequartista spot.
Gianluigi Buffon makes history today with his 903rd club football appearance, surpassing the record set by Paolo Maldini at Milan.
SPAL made the journey without Jasmin Kurtic, Federico Di Francesco, Marco D’Alessandro, Mattia Valoti or Mohamed Fares.
Igor comes into defence with Mirko Valdifiori, who worked with Sarri at both Empoli and Napoli, in midfield.
It was expected to be a choice between Alberto Paloschi and Sergio Floccari to act as partner for centre-forward Andrea Petagna, but Leonardo Semplici surprised everyone by picking 23-year-old Gabriele Moncini.
He only made one appearance for SPAL this season and that was in the Coppa Italia back in August.
Moncini did spend time in the Juventus youth academy, although last season he was at Cittadella.
Juve have failed to win either of their last two games played just before a Champions League tie, picking up a draw and a 2-1 defeat to SPAL in April.
Juventus: Buffon; Cuadrado, Bonucci, De Ligt, Matuidi; Khedira, Pjanic, Rabiot; Ramsey; Ronaldo, Dybala
Juventus bench: Szczesny, Pinsoglio, Rugani, Demiral, Beruatto, Emre Can, Bentancur, Higuain, Bernardeschi
SPAL: Berisha; Tomovic, Vicari, Igor; Sala, Murgia, Valdifiori, Missiroli, Reca; Moncini, Petagna
SPAL bench: Thiam, Letica, Cionek, Floccari, Strefezza, Felipe, Paloschi, Cannistra, Jankovic, Tunjov, Zanchetta
Watch Serie A live in the UK on Premier Sports for just £11.99 per month including live LaLiga, Eredivisie, Scottish Cup Football and more. Visit: https://subscribe.premiersports.tv/