Roma start Nikola Kalinic, Nicolò Zaniolo, Javier Pastore and Justin Kluivert in the top of the group showdown with Wolfsberger, who already beat Borussia Monchengladbach 4-0.
The Europa League tie kicks off in Austria at 17.55 UK time (16.55 GMT).
You can follow all the build-up and action as it happens from this game and Lazio-Rennes on the LIVEBLOG.
Both these sides got off to perfect starts in the Europa League with 4-0 victories, but while Roma were expected to beat Istanbul Basaksehir, it is somewhat surprising to see Wolfsberger earn a 4-0 result away to Borussia Monchengladbach.
Paulo Fonseca has some injury problems to deal with, as Lorenzo Pellegrini is out for at least two months after fracturing a bone in his foot, while Henrikh Mkhitaryan is sidelined for three weeks by a muscular problem.
Alessandro Florenzi, Cengiz Under and Diego Perotti are also on the treatment table, while Edin Dzeko and Aleksandar Kolarov are rested, so Nikola Kalinic gets his first start in a Roma jersey.
Zaniolo, Pastore and Kluivert are in support, with Amadou Diawara in midfield and Leonardo Spinazzola at left-back, but goalkeeper Antonio Mirante gets a rare appearance, as Pau Lopez is rested as a precaution due to muscular issues.
As for Wolfsberger, midfielder Mario Letigeb bagged a brace in the opener with Borussia Monchengladbach, while Marcel Ritzmaier and striker Shon Weissman also got on the scoresheet.
Anderson Niangbo and trequartista Michael Liendl complete the front line.
Wolfsberger: Kofler; Novak, Sollbauer, Rnic, Schmitz; Schmid, Leitgeb, Ritzmaier; Liendl; Weissman, Niangbo
Wolfsberger bench: Kuttin, Gollner, Schmidt, Sprangler, Baumgartner, Wemitznig, Schmerbock
Roma: Mirante; Santon, Fazio, Mancini, Spinazzola; Diawara, Cristante; Zaniolo, Pastore, Kluivert; Kalinic
Roma bench: Pau Lopez, Zappacosta, Juan Jesus, Dzeko, Kolarov, Veretout, Antonucci
Ref: Martins (POR)
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