Cristiano Ronaldo returns to the Juventus XI with Paulo Dybala and Aaron Ramsey, but Joao Felix is not ready to start for Atletico Madrid.
It kicks off at the Allianz Juventus Stadium in Turin at 20.00 GMT.
You can follow all the build-up and action as it happens from this game and Atalanta-Dinamo Zagreb on the LIVEBLOG.
The Bianconeri are already in the Round of 16, but a win here would guarantee top spot in the group and in theory an easier draw for the next round.
Atleti are not through yet and hit problems after their defeat to Bayer Leverkusen.
When these sides last met at the Wanda Metropolitano, it ended 2-2 with a late Colchoneros comeback from 2-0 up.
They also caused huge problems last season when a Ronaldo hat-trick in the second leg completed the turnaround.
Ronaldo skipped Saturday’s 3-1 Serie A victory away to Atalanta, as he was rested due to an ongoing knee issue after international duty, but returns to the starting XI with Dybala, sending Gonzalo Higuain to the bench.
Ramsey gets the nod as the trequartista rather than Federico Bernardeschi, while Matthijs de Ligt can start despite dislocating his shoulder at the weekend.
Danilo and Mattia De Sciglio are the full-backs, resting Juan Cuadrado.
Douglas Costa, Alex Sandro, Adrien Rabiot and Giorgio Chiellini are out injured, with Emre Can and Mario Mandzukic not in the Champions League squad.
Atletico Madrid had to wait until the last minute for a fitness test on their €126m starlet Joao Felix, as since dislocating his ankle on October 19, he made only one 15-minute appearance at the weekend in the 1-1 draw with Granada.
The Portugal international youngster is not considered ready to start, so former Juventus striker Alvaro Morata is instead partnered y Vitolo.
Saul Niguez replaces Angel Correa and Thomas Partey gets the nod rather than Marcos Llorente.
Atleti have made two previous trips to Turin, failing to score a single goal in their 0-0 draw and last season’s 3-0 defeat.
The referee is Anthony Taylor with fellow Englishman Stuart Attwell in the VAR booth.
Juventus: Szczesny; Danilo, Bonucci, De Ligt, De Sciglio; Bentancur, Pjanic, Matuidi; Ramsey; Cristiano Ronaldo, Dybala
Juventus bench: Buffon, Rugani, Demiral, Khedira, Cuadrado, Higuain, Bernardeschi
Atletico Madrid: Oblak; Trippier, Felipe, Hermoso, Renan Lodi; Saul Niguez, Herrera, Thomas, Koke; Morata, Vitolo
Atletico Madrid bench: Adan, Arias, Joao Felix, Correa, Lemar, Llorente, Saponjic
Ref: Taylor (ENG)
Watch Serie A live in the UK on Premier Sports for just £9.99 per month including live LaLiga, Eredivisie, Scottish Cup Football and more. Visit: https://www.premiersports.com/subscribenow